Department of Animal Biotechnology
Christopher Porada

Photo of Christopher Porada

Associate Professor
Department of Animal Biotechnology
University of Nevada/Mail Stop 202
1664 North Virginia Street
Reno,  Nevada   89557

 

Office: (775) 784-6750
Lab: 784-8048

Fax: 784-1745

Email: cporada@cabnr.unr.edu
Building: Max Fleischmann Agriculture,  Office N/A
Personal Web: http://www.cabnr.unr.edu/Porada/

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EDUCATION

B.A., Molecular Biology, 1991, Colgate University
Ph.D., Cellular & Molecular Pharmacology and Physiology, 1998, University of Nevada, Reno

 

ACADEMIC & RESEARCH INTEREST

My research interests are focused on developing methods of safely and efficiently performing gene therapy before to birth, thus allowing correction of genetic disorders prior to the onset of disease and resultant tissue damage.  Approaches being explored are systems to allow targeting of specific cell types following direct administration of viral-based gene delivery vectors to the fetus and using genetically modified stem cells as gene delivery vehicles.  These technologies are then being tested in a preclinical model of the bleeding disorder Hemophilia A.

 
COURSES & TEACHING RESPONSIBILITIES

ANSC 101 - Introduction to Animal Biotechnology, 2 credits

ANSC 415/615 - Biotechnology: Economic and Ethical Issues (A Capstone Course), 3 credits

ANSC 428/628 - Stem Cell Biology, 3 credits

ANSC 429/729 - Biotechniques/Advanced Biotechniques, 3 credits

CMB 794 - Colloquium Gene Regulation, 1 credit

 
REPRESENTATIVE PUBLICATIONS

 

Journals
Yamagami T, Porada CD, Pardini RS, Zanjani ED, Almeida-Porada G. 2009, "Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell line." Cancer Biol Ther. 8(4), 2009.  
Park PJ, Colletti E, Ozturk F, Wood JA, Tellez J, Almeida-Porada G, Porada C. 2009, "Factors Determining the Risk of Inadvertent Retroviral Transduction of Male Germ Cells Following in Utero Gene Transfer in Sheep." Hum Gene Ther. 2008 Nov 26. [Epub ahead of print]  
Porada C.D., Harrison-Findik D.D., Sanada S., Valiente V., Thain D., Simmons P.J., Almeida-Porada G., Zanjani E.D. 2008, "Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells." Experimental Hematology, 36(12):1739-49, 2008.  
Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD. 2008, "Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction." Exp Hematol. 2008 Jul;36(7):816-22.  
Frias AM, Porada CD, Crapnell KB, Cabral JM, Zanjani ED, Almeida-Porada G. 2008, "Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion." Exp Hematol. 36(1):61-8.  
Chamberlain J, Yamagami T, Colletti E, Theise ND, Desai J, Frias A, Pixley J, Zanjani ED, Porada CD, Almeida-Porada G. 2007, "Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep." Hepatology. 46(6):1935-45.  
Almeida-Porada G, Porada C, Gupta N, Torabi A, Thain D, Zanjani ED. 2007, "The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential." Exp Hematol. 35(10):1594-600.  
Porada CD, Zanjani ED, Almeida-Porada G. 2006, "Adult mesenchymal stem cells: a pluripotent population with multiple applications." Curr Stem Cell Res Ther. 1(3):365-9.  
Porada, C.D., Park, P.J., Almeida-Porada, G., Liu, W., Ozturk, F., Glimp, H.A., Zanjani, E.D. 2005, ""Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer."" Molecular Therapy 11(2): 284-293.  
Porada, C.D., Park, P.J., Tellez, J., Ozturk, F., Glimp, H.A., Almeida-Porada, G., Zanjani, E.D. 2005, ""Male germline cells are at risk following direct injection retroviral-mediated gene transfer in utero."" Molecular Therapy 12(4):754-62, 2005.  
Lucas ML, Seidel NE, Porada CD, Quigley JG, Anderson SM, Malech HL, Abkowitz JL, Zanjani ED, Bodine DM. 2005, "Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopes." Blood. 106(1):51-8.  
Almeida-Porada G., Porada, C.D., Torabi A., Zanjani, E.D. 2004, "Formation of human hepatocytes by human hematopoietic stem cells in sheep." Blood 104(8):2582-90  
Almeida-Porada G., Porada, C.D., Torabi A., Zanjani, E.D. 2004, "Formation of human hepatocytes by human hematopoietic stem cells in sheep." Blood 104(8):2582-90  
Almeida-Porada, G., Porada, C., Zanjani, E.D. 2004, "Plasticity of Human Stem Cells in the Fetal Sheep Model of Human Stem Cell Transplantation. I" Int’l J. Hem. 79:1-6  
Porada C.D., Park P., Almeida-Porada G., Zanjani E.D. 2004, "The fetal sheep model of in utero gene therapy." Fetal Diagnosis and Therapy 19(1):23-30  
Tsai EJ, Malech HL, Kirby MR, Hsu AP, Seidel NE, Porada CD, Zanjani ED, Bodine DM, Puck JM. 2002, "Retroviral transduction of IL2RG into CD34(+) cells from X-linked severe combined immunodeficiency patients permits human T- and B-cell development in sheep chimeras." Blood. 100(1):72-9.  
Porada CD, Tran ND, Almeida-Porada G, Glimp HA, Pixley JS, Zhao Y, Anderson WF, Zanjani ED. 2002, "Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors." Hum Gene Ther. 2002 May 1;13(7):867-79.