Minutes of the 1999-2000 Annual Meeting of W-181
Modifying Milk Fat Composition for Improved Manufacturing Qualities and
Consumer Acceptability
January 10- 11, 2000 Reno, NV
Individuals attending
Denise Beaulieu (University of Illinois)
Ed Depeters (University of California - Davis)
Francois Enjalbert (France)
Tilak Dhiman (Utah State University)
Jim Drackley ((University of Illinois)
Mikko Griinari (Finland)
Tom Jenkins (Clemson University, South Carolina)
Cindy Luhman (Land O'Lakes, Webster City, IA)
Mark McGuire (University of Idaho)
Don Palmquist (Ohio State University)
Dave Schingoethe (South Dakota University)
Dale Bauman (Cornell University)
Sharon T. Franklin (University of Kentucky)
Hussein S. Hussein (University of Nevada)
Stanley T. Omaye (University of Nevada)
Beverly B. Teter (University of Maryland)
Scott Naemi (Land O'Lakes, Webster City, IA)
Ron Pardini (University of Nevada-Reno, Administrative Advisor)
Henry F. Tyrrell (CSREES Representative)
Monday morning, January 10, 2000 The meeting was called to order by the
chairman, Mark McGuire, at 8:25 a.m.
Secretary's report
The first order of business was the introduction of the
Committee members. Dr.Ron Pardini welcomed the members to Reno. Dr. Pardini
provided a brief insight on the changes in the operations of multi-state
research projects. He mentioned that in the future there would be less paper
work because most of the things will be handled on the internet. Dr. Pardini
congratulated members on project renewal. Dr. Don Palmquist mentioned that the
project was renewed with very few changes.
M/S Dr. Tom Jenkins/ Dr. Beverly Teter to approve minutes of
the 1999-2000 meeting. Motion carried.
Dr. Mark McGuire distributed a copy of an article
"Fractionated milk fat composition, structure, and functional
properties" from Kerry Kaylegian, Wisconsin Center for Dairy
Research to the committee members. The summary of this
article was that variability in functionality of milk fat can be minimized by
fractionation and this will enhance its functionality in confectionery and baked
products.
Food labeling regulations for fat content of foods by the FDA
was discussed. Dr. Palmquist shared proposed rules on food fat labeling. The
recommended limit for listing saturated fat content of foods will not change.
However, declaration is required for any fat that provides 0.5 g or greater of
trans fatty acids per serving. Assuming 3% total trans fatty acids in milk, the
recommended daily allowance of milk and cheese for humans would supply less than
0.5 g of trans fatty acids. Dr. Teter distributed calculations on trans fatty
acids in dairy products to the committee members. She commented that dairy
products are not affected by the declaration rule of 0.5 g trans fatty acids
even if the consumption is doubled. Dr. Palmquist asked the opinion of committee
members on this rule. The following two points emerged from the discussion: 1.
All trans fatty acids are not created equal. 2. The trans fatty acids should be
reported on a separate line and not be added to the saturated fatty acids. Dr. Griinari, Dr. McGuire and Dr. Teter pointed out that different isomers of trans
fatty acids have different physiological effects. Dr. Bauman responded to this
comment by suggesting that a letter should be drafted to FDA as a response from
the W-181 committee members.
Dr. McGuire distributed an article "Fatty acid profiles
needed for dairy foods from the United States" written by Dr. Robert
Jensen. Dr. Robert Jensen could not attend the meeting because of health
problems. Dr. McGuire reported that CLA content of milk varies among cows, and
committee members as a group should study the variation among cows in CLA
content of milk. Dr. McGuire requested committee members to send him milk
samples for CLA analysis.
Administrative remarks:
Dr. Ron Pardini noted that Extension and Research has
to spend 25% of the Federal research dollars on multi-state research projects.
The input from stakeholders into the research project is important. There will
be more regional input on national research projects than before. The
researchers have to write an impact statement for their project Dr. Pardini
encouraged the committee members to submit an impact statement from each
project. There will be a national impact statement database called Impact-2000.
Dr. Henry F. Tyrell (CSREES Representative) mentioned
that the new farm bill ha: brought some changes in the operation of regional
research projects and the Western Regional Project is ahead in making these
changes. Some of these changes are a; mentioned above by Dr. Pardini; that 25%
of the Hatch funding has to be spent or multi-state activities. There is more
research funding for integrated activities in the area of water quality and food
safety. Last year agriculture received the largest increase it research
funding. Estimates of ammonia in the environment indicate that 90% of ammonia
comes from livestock and therefore animal agriculture can be considered a point
source of ammonia emissions. After the year 2002, the EPA will regulate ammonia
emissions. Dr. Bauman pointed out that we need science behind these regulations.
Dr Tyrell responded that EPA is under
pressure from the non-scientific community and it has to make a decision by
2002. Dr. Tyrell said that the W-181 is the star among regional projects and he
praised the members for their efforts.
Station reports:
University of California, Dr. Edward DePeters discussed the
effect of desaturase genotype on milk fatty acid composition. Polymorphism was
found in the stearoyl CoA desaturase (SCD) gene. Variants were identified as AA,
AB, and AB. Cows of the AA genotype for the SCD had higher C 18:0 fatty acid in
milk fat compared with AB and BB cows. In two subsequent studies, genotype for
SCD had little effect on fatty acid composition of milk fat. Results suggest
that polymorphism had no influence on fatty acid composition.
Cornell University, Dr. Bauman reported that feeding full fat
extruded soybeans increased the concentration of conjugated linoleic acid (CLA)
in milk fat.
Clemson University, South Carolina, Dr. Tom Jenkins discussed
effects of oleamide on feed intake and ruminal fatty acid concentrations.
Addition of oleamide at a level greater more than 1 % of dietary DM reduced the
feed intake of animals. The reduction in feed intake when oleamide is added to
the diet can be attributed more to physiological responses than palatability,
and the intake depression caused by oleamide lessens over time.
Cornell University, Dr. Bauman discussed the role of products
of rumen fermentation on milk fat depression (MFD). Two conditions are required
to get MFD - a dietary source of unsaturated fatty acids and an altered rumen
fermentation that leads to incomplete biohydrogenation. MFD was related to a
shift in the pattern of trans C 18:1 isomers resulting in increased
concentrations of traps-10 C18:1. Based on research results, Dr. Bauman
postulated that traps-10 C18:1 or related metabolites (traps-10 cis- 12 CLA)
might be causes of MFD. Abomasal infusion of traps10, cis-12 CLA resulted in
40% reduction in milk fat content and yield. Currently, Dr. Bauman is
investigating the mechanisms whereby traps-10, cis- 12 CLA inhibits milk fat
synthesis.
Dr. Bauman also noted that initial studies suggest that cows
have the capacity to convert traps11 C 18:1 to cis-9, traps-11 CLA by
endogenous synthesis. The CLA can also be concentrated in milk fat by certain
food manufacturing techniques such as the continuous supercritical carbon
dioxide system. Concentration was increased by 2.5-fold.
Dr. Bauman also reported results from a study investigating
the biological effects of different CLA isomers. This study demonstrated that
dairy products containing natural
CLA were effective anticarcinogens, and that cis-9, trans- 11
CLA isomer possesses the anticarcinogenic effect.
France, Dr. Enjalbert reported effects of a wider range of
arterial concentrations of trans fatty acids on mammary metabolism and milk
composition. Stearic, oleic and linoleic acids were used to obtain wide
variation in trans fatty acids. There was a significant positive relationship
between trans 11 C 18:1 and cis-9, trans- 11 C 18:2, but there was no
relationship between trans- 10 C18:1 and trans- 10, cis- 12 C18:2.
University of Idaho, Dr. Mark McGuire reported results from a
study aimed at increasing CLA in milk by feeding calcium salts of a commercially
available CLA mixture or by infusing them into the abomasum. The protected CLA
was fed at 50g/d per head. Feeding or infusing protected CLA into the abomasum
decreased milk fat content. The concentrations of cis-9, trans-12 CLA were
doubled, and concentrations of other CLA isomers present in the CLA mixture were
also detected in milk fat. In another study, concentrations of CLA were 4 to 5
mg/g of fat in meat from deer, elk, and moose but were greater in meat from
mountain lions (7 mg/g of fat) and greatest in meat from black bears (9 mg/g of
fat).
University of Illinois, Dr. Drackley and Dr. Beaulieu
reported effects of post ruminal infusion of unsaturated fatty acids as
triglycerides or free fatty acids on feed intake, milk production and milk fat
synthesis. Both lipid sources linearly decreased feed intake. The free fatty
acids were 2.5 times more potent in decreasing feed intake than triglycerides.
Milk fat content was increased linearly as the amount of fat infused was
increased. Effects of feed restriction on milk components and fatty acid
composition were also reported. The results indicate that changes in proportions
of different fatty acids in milk fat with abomasal infusion of unsaturated fatty
acids may be due to decreased feed intake. However, feed restriction did not
influence the particle size of milk fat. In another study feeding soybean oil at
4% of dietary DM increased the CLA content of milk.
Comments on the response to FDA on labeling of fat content of foods:
Dr. Beverly Teter and Dr. Don Pamquist shared the draft of a
letter to the Food and Drug Administration and asked committee members' input.
There were three major points to this letter. 1. All trans fatty acids are
metabolized differently and have different physiological effects; 2. Trans fatty
acids should be labeled separately; 3. Fatty acids less than 12 carbon should
not be included as saturated fat on food label. This letter will be signed by
all committee members and sent to the Food and Drug Administration, Rockville,
MD before February 15, 2000.
Land O'Lakes, Iowa, Dr. Cindie Luhman discussed effects of
feeding CLA on cow performance. Feeding CLA as oil at 0.5, 1.0 or 1.5 lb. per
cow/day reduced feed intake and decreased
milk yield. In another study feeding soybean oil and sunflower oil at 2 lb. per
day/cow increased the CLA content of milk.
OARDC/The Ohio State University, Dr. Don Palmquist discussed
the effects of feeding roasted soybeans on the development of oxidized flavor of
milk. Feeding roasted soybeans increased the polyunsaturated fatty acid content
in milk and increased the susceptibility of milk to spontaneous oxidation. The
presence of high copper increased the severity of oxidized flavor. These factors
accounted for one-half the variability in oxidized flavor score after storage
for 8 days.
South Dakota State University, Dr. D. J. Schingoethe reported
the effects of feeding fish oil at 1, 2, and 3% of dietary DM on fatty acid
composition of milk, including CLA. Feeding fish oil at 2 and 3% of the dietary
DM reduced feed intake and milk yield. Adding fish oil decreased milk fat
content and enhanced the concentrations of CLA, and omega-3 fatty acids in milk
fat. In another study feeding fishmeal to lactating dairy cows increased the CLA
content of milk. Dr. Schingoethe also discussed the effects of feeding fish oil
on the physical and chemical properties of milk and butter. Milk flavor was
similar and acceptable for milk from cows fed diets containing 0 and 2% fish
oil.
Utah State University, Dr. Tilak R. Dhiman reported effects
of CLA enriched cheese on body composition of mice. Mice were fed diets
containing low CLA cheese, medium CLA cheese, high CLA cheese or low CLA cheese
plus a mixture of synthetic CLA. The CLA contents of diets were 0.12, 0.33,
0.53, and 0.53 mg/g of fat in low, medium, high and synthetic CLA treatments.
Feeding CLA enriched cheese did not influence the body composition of mice.
Feeding synthetic CLA to mice reduced body fat by 57% compared with mice fed
high CLA cheese.
Election of Secretary
M/S Dr. Drackley/ Dr. McGuire to nominate Dr. Cindie Luhman
for secretary. Motion passed. After her term as secretary, Dr. Luhman will serve
as Chair in 2002.
Date and Location of Meeting
The next meeting is schedule for January 8 - 9, 2001, in
Reno.
Other business